Journal: International Journal of Molecular Sciences

Article Title: Neuronal Differentiation and Exosome Profiling of Dental Pulp Stem Cells: Unveiling Their Potential for Nerve Repair

doi: 10.3390/ijms26199723

Figure Lengend Snippet: ∆Ct values for the different genes under study in hDPSCs, undifferentiated and after neurogenic differentiation. Higher ∆Ct values represent lower expression. Results are presented as mean ± SEM.

Article Snippet: Media was removed from all the wells, and the neurogenic differentiation medium (MSC Differentiation Medium, PromoCell ® , Heidelberg, Germany) was allocated in 8 wells, while the remaining 2 wells were used as controls and maintained with the usual culture medium.

Techniques: Expressing

Journal: BioMedical Engineering OnLine

Article Title: Endothelial cells influence the osteogenic potential of bone marrow stromal cells

doi: 10.1186/1475-925X-8-34

Figure Lengend Snippet: mRNA Expression after 5 days by real time PCR . There were four groups: Group 1: MSC co-cultured with EC (Co); Group 2: MSC induced by osteogenic stimulatory medium (OM); Group 3: MSC in mono-culture without osteogenic stimulatory supplements (MSC) and Group 4: EC only (EC). Results were reported as fold changes ± fold-difference (2 -ΔΔCt ± 2 -(ΔΔCt ± SD) ). The y-axis showed mRNA level in logarithmic scale for ALP, RunX2 and Col I. A significant upregulation of ALP expression was found in MSC/EC co-cultures compared with MSC alone. All the data were normalized with endogenous control GAPDH. ** P < 0.01 * P < 0.05.

Article Snippet: In order to confirm the Superarray results, real-time PCR was performed independently and 5 day osteogenic stimulatory medium (OM)-induced MSC were used as a positive control (Fig. ).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture, Control

Journal: Micromachines

Article Title: Fabrication of a Cell-Friendly Poly(dimethylsiloxane) Culture Surface via Polydopamine Coating

doi: 10.3390/mi13071122

Figure Lengend Snippet: Staining results showing MSC differentiation after 21 days of culture. ( a ) Osteogenic and ( b ) chondrogenic differentiation was evaluated by staining with Alizarin Red S and Safranin O, respectively. The dotted circles and ellipses represent the same spot in the images. Scale bar = 500 μm.

Article Snippet: MSCs, HUVECs, MSC growth medium (MSCM), endothelial cell growth medium (ECM), endothelial cell growth supplements (ECGS), MSC growth supplements (MSCGS), fetal bovine serum (FBS), penicillin/streptomycin (P/S) solution, trypsin-EDTA (T/E) solution, Dulbecco’s phosphate-buffered saline (DPBS), MSC chondrogenic differentiation medium (MCDM), MSC chondrogenic differentiation supplement (MCDS), MSC osteogenic differentiation medium (MODM), and MSC osteogenic differentiation supplement A and B (MODS-A and MODS-B) were purchased from ScienCell (Carlsbad, CA, USA).

Techniques: Staining

Journal: Frontiers in Endocrinology

Article Title: Extracellular Vesicles Inhibit Proliferation and Invasion of Ovarian Endometrial Stromal Cells and Their Expression of SF-1, ERβ, and Aromatase

doi: 10.3389/fendo.2021.666195

Figure Lengend Snippet: Identification of UC-MSCs. (A) UC-MSCs were exposed to adipogenic differentiation medium and stained by Oil Red O. Compared to cells in the control group, the red, round lipid droplets observed in UC-MSCs treated with adipogenic differentiation medium confirms their differentiation into adipocytes. (B) UC-MSCs were cultured with osteoblast differentiation medium and stained by Alizarin Red S. As evidenced by the red round droplet in the righthand image, UC-MSCs could differentiate into osteocytes. (C) Stem cell phenotypes were identified by flow cytometry. Positive indicators (CD90, CD105, CD44, and CD29) showed that the cells to be tested were stem cells rather than ordinary cells. In addition, negative markers (CD19 and HLA-DR) showed that the cells to be tested were mesenchymal stem cells rather than hematopoietic stem cells. All experiments were repeated three times.

Article Snippet: MSCs were seeded in 6-well plates at 10 5 cells/well, and then cultured in osteoblast medium (50 mL osteoblast MSCM + 5 mL FBS + 0.5 mL double antibody + 0.5 mL MSCGS; Sciencell, MSC osteogenic differentiation medium, 7531, USA) after growth and fusion.

Techniques: Staining, Control, Cell Culture, Flow Cytometry